genotyping of infectious bronchitis viruses in the east of iran, 2015

background: infectious bronchitis virus (ibv) is the causative agent of avian infectious bronchitis, which is characterized by respiratory, reproductive, and renal signs. ibv is a highly variable virus with a large number of genotypes. s1 gene sequencing has been used for molecular epidemiological studies and genotypic characterization of ibv. objective: to better understand the molecular epidemiology of ibv in iran, we sequenced the s1 gene of  ibv field isolates, a total of 40 tracheal and kidney tissue specimens from different commercial broiler flocks in the east of iran were collected from 2015. methods: 15 ibv-positive samples were selected from among the total and were further characterized by sequencing the spike glycoprotein gene.   the isolates were confirmed by real-time polymerase chain reaction (pcr) and  characterized by sequencing the spike glycoprotein gene. results: three genotypes were detected. the percentage of variant 2 (is/1494 like), 793/b, and qx genotypes was 66.7%, 26.7%, and 6.6% respectively. we reported the qx as the first time and variant2 was the dominant genotype in this area .conclusion: it is an updated and comprehensive study of genotyping of ibv and completes ibv puzzle in the east of iran.